Turnip yellow mosaic virus variants produced from DNA clones encoding their genomes
Identifieur interne : 004793 ( Main/Exploration ); précédent : 004792; suivant : 004794Turnip yellow mosaic virus variants produced from DNA clones encoding their genomes
Auteurs : M. L. Skotnicki [Australie] ; A. M. Mackenzie [Australie] ; A. J. Gibbs [Australie]Source :
- Archives of Virology [ 0304-8608 ] ; 1992-03-01.
English descriptors
- Teeft :
- Amino acid sequence, Blue lake, Cabbage, Cdna, Chinese cabbage, Chinese cabbage plants, Chinese cabbages, Clone, Club lake, Cytosine, Cytosine content, Cytosine residues, Endonuclease, Extra nucleotides, Genome, Genomic, Hybrid, Hybrid clones, Hydrolysed, Inoculated, Mosaic, Mosaic virus, Mutant, Nucleotide, Plant viruses, Plasmid, Polymerase, Primer, Proc natl acad, Restriction endonuclease, Skotnicki, Symptom, Symptom differences, Synthetic primer, Systemic symptoms, Systemically, Terminus, Tobacco mosaic virus, Turnip, Tycless, Tymoviruses, Tymv, Tymv mutants, Universal primer, Viral, Viral genome, Viral inserts, Viral sequence, Virion, Virion protein, Virion protein gene.
Abstract
Summary: Full-length dsDNA clones that encode the genomes of two Australian turnip yellow mosaic isolates, TYMV-BL and TYMV-CL have been constructed. These clones were transcribed to give 6.3 kb capped ssRNA which infects Chinese cabbages to give symptoms indistinguishable from those produced by the parental viruses. Extensions of up to 26 nucleotides at the 3′ end of the TYMV-BL clone delay infections, but virus particles isolated from these plants 4 weeks after inoculation contain RNA with the original TYMV-BL 3′ terminus. A 90 nucleotide-long portion of the virion protein gene of TYMV-BL was replaced by a synthetic 90-mer primer with 16 nucleotide changes to decrease the large cytosine content (34–42%) characteristic of tymovirus genomic RNA. No reversion of any of the mutated nucleotides to cytosine occurred during 7 passages in Chinese cabbage. Hybrids between the TYMV-BL and TYMV-CL clones were also constructed, by exchanging various portions of the genome. However, it was not possible to determine definitively which part of the viral genome is responsible for the more severe symptoms caused by TYMV-BL as the hybrids gave intermediate symptoms.
Url:
DOI: 10.1007/BF01309572
Affiliations:
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These clones were transcribed to give 6.3 kb capped ssRNA which infects Chinese cabbages to give symptoms indistinguishable from those produced by the parental viruses. Extensions of up to 26 nucleotides at the 3′ end of the TYMV-BL clone delay infections, but virus particles isolated from these plants 4 weeks after inoculation contain RNA with the original TYMV-BL 3′ terminus. A 90 nucleotide-long portion of the virion protein gene of TYMV-BL was replaced by a synthetic 90-mer primer with 16 nucleotide changes to decrease the large cytosine content (34–42%) characteristic of tymovirus genomic RNA. No reversion of any of the mutated nucleotides to cytosine occurred during 7 passages in Chinese cabbage. Hybrids between the TYMV-BL and TYMV-CL clones were also constructed, by exchanging various portions of the genome. However, it was not possible to determine definitively which part of the viral genome is responsible for the more severe symptoms caused by TYMV-BL as the hybrids gave intermediate symptoms.</div></front></TEI><affiliations><list><country><li>Australie</li></country></list><tree><country name="Australie"><noRegion><name sortKey="Skotnicki, M L" sort="Skotnicki, M L" uniqKey="Skotnicki M" first="M. L." last="Skotnicki">M. L. Skotnicki</name></noRegion><name sortKey="Gibbs, A J" sort="Gibbs, A J" uniqKey="Gibbs A" first="A. J." last="Gibbs">A. J. Gibbs</name><name sortKey="Mackenzie, A M" sort="Mackenzie, A M" uniqKey="Mackenzie A" first="A. M." last="Mackenzie">A. M. Mackenzie</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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